This list misses some of the best:
Farah from PoP. If she isn't dying, she's accidentaly shooting you.
Sylvie Christel in No More Heroes. All of her speeches are the same and are annoying and can't be skipped. Her cellphone soliloquies all follow the pattern "You're about to fight so you should use the restroom, you're definately going to die this time [OK THIS time you're gonna die for real], but trust your force and head for the garden of madness"
R2-D2 in any star wars game game featuring his "WHHEEEOOOWWW!!!" 'Nuff said.
Dry Bones. They're like weebls, weebls wobble but they don't fall down. I just want them to die, but by the magic of levitation they manage to put themselves back together.
Every "character" in a DDR game. etc.