the FIRST scary moment for me was the invisible novistadors in the sewers
and the one true scary moment was the regenerator lying on that autopsy table o.o
...and i guess when looking at the iron maiden on the TV in the lab
i also like to scare myself when i play ashley's adventure; you wait by the exit and wait until the three knights come RIGHT in front of you! =O then you press 'A'
something else i found on youtube, u can run right up to the last regenerater in the prison and he wont notice you! so i tried that and it was freaky just standing an inch away from a live regenerator xD he was just standing there looking to his left and right...